Crystal Violet Solution for the Gram Stain

This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures. Crystal Violet Ingredients 2 g crystal violet20 ml 95% ethyl alcohol0.8 g ammonium citrate monohydrate80 ml deionized water Prepare Crystal Violet Stain Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.